羟基喜树碱抑制人眼Tenon囊成纤维细胞增殖的实验研究

目的:研究羟基喜树碱(HCPT)对体外培养的人眼Tenon囊成纤维细胞(Human Tenon’s capsule fibroblasts,HTFs)增殖、移行的影响。方法:取正常供体新鲜的Tenon囊组织,采用组织块培养法,进行成纤维细胞的体外培养,并用光镜、免疫荧光法观察鉴定;MTT法、划痕法检测不同浓度的HCPT(0、0.031、0.062、0.125、0.25、0.5、1、2、4mg/l)对HTFs增殖、移行的影响,并与MMC对比。结果:HTFs体外生长良好,经光镜和免疫荧光法观察鉴定为成纤维细胞;与空白对照组比较,HCPT(0.031-4mg/l)、MMC(0.0031-0.4mg/l)均能有效抑制HTFs的增殖,且呈一定的剂量、时间依赖性,HCPT作用24h、48h、72h的IC50分别为2.24mg/l、0.76mg/l、0.39mg/l,MMC作用24h、48h、72h的IC50分别为0.34mg/l、0.24mg/l、0.07mg/l;与空白对照组比较,HCPT(0.031-4mg/1)、MMC(0.0031-0.4mg/l)均能抑制HTFs迁移,呈剂量依赖性,而与时间无显著相关。结论:HCPT、MMC均能有效抑制HTFs的增殖和移行,其效应MMC约为HCPT的10倍。

Inhibitory Effect of 10-Hydroxycamptothecin on the Cultured Human Tenon's Capsule Fibroblasts

Objective:To investigate the effect of 10-hydroxycamptothecin on the proliferation,migration of human Tenon's capsule fibroblasts.Methods:Fresh donor of normal Tenon capsule,cultured fibroblasts in vitro using tissue culture were obtained.HTFs was detected by using light microscopy,immunofluorescence;The inhibitory effect of HCPT at different concentrations on HTFs (0,0.031,0.062,0.125,0.25,0.5,1,2,4mg/l) was measured by methylthiazlyltetrazolium(MTT) assay;Scratch assays were used to evaluate the migration...