| 赫赛汀联合姜黄素对卵巢癌细胞系SKOV3的抑制作用 |
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| 引用本文： | 司马玲,姜群英,刘彩霞,冯静,冯桂萍.赫赛汀联合姜黄素对卵巢癌细胞系SKOV3的抑制作用[J].现代生物医学进展,2011,11(16):3080-3083. |
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| 作者姓名： | 司马玲 姜群英 刘彩霞 冯静 冯桂萍 |
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| 作者单位： | 兰州军区乌鲁木齐总医院妇产科 新疆乌鲁木齐 830000 |
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| 摘 要： | 目的:探讨赫赛汀联合姜黄素对HER2阳性卵巢癌细胞系SKOV3增殖能力的影响。方法:体外培养至对数生长期的人卵巢癌细胞系SKOV3细胞分为对照组、赫赛汀治疗组、姜黄素治疗组以及赫赛汀联合黄素治疗组,利用四唑盐(MTT)比色法和平板集落形成试验比较各组细胞增殖能力,利用AnnexinV-FITC/PI染色比较各组细胞凋亡。结果:赫赛汀联合姜黄素治疗组SKOV3细胞活力明显低于对照组及单一药物处理组,平板集落形成试验结果表明各组细胞集落形成率分别为83.4%、36.8%、59.2%和24.7%,赫赛汀联合姜黄素处理组SKOV3细胞集落形成能力显著低于另外3组(P<0.01),Annexin V-FITC/PI染色并用流式细胞仪分析表明各组SKOV3细胞早期凋亡率分别为1.3%、26.4%、9.3%和39.1%,赫赛汀联合姜黄素处理组细胞早期凋亡率显著低于其余3组(P<0.01)。结论:赫赛汀联合姜黄素能够抑制卵巢癌细胞系SKOV3的增殖,这种抑制作用是通过促进肿瘤细胞凋亡实现的。
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| 关 键 词： | 姜黄素 赫赛汀 卵巢癌 凋亡 |
Synergistic Inhibitory Effect of Curcumin Combined with Herceptin on Human Ovarian Cancer Cell Line SKOV3 |
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| SI-MA Ling,JIANG qun-ying,LIU Cai-xia,FENG Jing,FENG Gui-ping.Synergistic Inhibitory Effect of Curcumin Combined with Herceptin on Human Ovarian Cancer Cell Line SKOV3[J].Progress in Modern Biomedicine,2011,11(16):3080-3083. |
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| Authors: | SI-MA Ling JIANG qun-ying LIU Cai-xia FENG Jing FENG Gui-ping |
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| Institution: | Δ(Department of Gynecology-Obstetrics,Urumchi Hospital of Lanzhou Military region,Urumchi 830000,China ) |
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| Abstract: | Objective:To investigate the effects of Herceptin combined with curcumin on human ovarian cancer cell line SKOV3 in vitro.Methods:SKOV3 cells were treated with Herceptin,curcumin,Herceptin and curcumin,respectively.Cell viability was tested by MTT assay and colony-forming assay.Results:Cell viability of SKOV3 cells treated by Herceptin combined with curcumin was lower than that of Herceptin or curcumin alone.The colony forming efficiency of each group cells was 83.4%,36.8%,59.2%and 24.7%, respectively(P<0.0... |
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| Keywords: | Herceptin Curcumin Ovarian cancer SKOV3 Apoptosis |
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