一株高浓度多环芳烃降解菌的鉴定和降解特性

采用选择性富集培养方法,从沈抚灌区土壤中分离得到多环芳烃(PAHs)高效降解菌NI2,应用此降解菌制备固定化菌剂,修复焦化厂内高浓度PAHs污染土壤,并通过生理生化和16S rDNA测序进行微生物鉴定.经过30 d的降解实验,菌N12对污染土壤中各PAH的去除率＞66％,总去除率为80％.生理生化和16S rDNA测序分析表明,分离得到的菌株N12为分支杆菌属(Mycobacterium sp.),该菌具有与其他分枝杆菌同源的双加氧酶基因nidA和pdoA2.结果表明,从土壤中筛选获得的分枝杆菌可以修复高浓度PAHs污染工业土壤.

Identification and degradation characteristics of a bacteria strain degrading high concentration of polycyclic aromatic hydrocarbons

By the method of selective enrichment culture,a high efficient polycyclic aromatic hydrocarbons(PAHs)-degrading bacterial strain N12 was isolated from a soil collected from Shenfu Irrigation Area,Liaoning Province,and was immobilized as a microbial agent to remedy the high concentration PAHs-polluted soil in a coking plant.Based on the physiological and biochemical analysis and 16S rRNA sequencing,the N12 was identified as Mycobacterium sp.,with the isogenous dioxygenase genes nidA and pdoA2 of other Mycoba...