Statistical optimization of alkaline xylanase production from <Emphasis Type="Italic">Streptomyces violaceoruber</Emphasis> under submerged fermentation using response surface methodology |
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Authors: | S Khurana M Kapoor S Gupta R C Kuhad |
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Institution: | (1) Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110 021, India |
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Abstract: | Response surface methodology employing central composite design (CCD) was used to optimize fermentation medium for the production
of cellulase-free, alkaline xylanase from Streptomyces violaceoruber under submerged fermentation. The design was employed by selecting wheat bran, peptone, beef extract, incubation time and
agitation as model factors. A second-order quadratic model and response surface method showed that the optimum conditions
for xylanase production (wheat bran 3.5 % (w/v), peptone 0.8 % (w/v), beef extract 0.8 % (w/v), incubation time 36 h and agitation
250 rpm) results in 3.0-fold improvement in alkaline xylanase production (1500.0 IUml−1) as compared to initial level (500.0 IUml−1) after 36 h of fermentation, whereas its value predicted by the quadratic model was 1347 IUml−1. Analysis of variance (ANOVA) showed a high coefficient of determination (R2) value of 0.9718, ensuring a satisfactory adjustment of the quadratic model with the experimental data.
The economical and cellulase-free nature of xylanase would enhance its applicability in pulp and paper industry. |
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Keywords: | Alkaline CCD RSM Streptomyces violaceoruber Xylanase |
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