| MTT比色法检测赖氨酸、蛋氨酸对体外培养的奶牛乳腺上皮细胞增殖的影响 |
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| 引用本文： | 李喜艳,王加启,魏宏阳,卜登攀,胡菡,周凌云.MTT比色法检测赖氨酸、蛋氨酸对体外培养的奶牛乳腺上皮细胞增殖的影响[J].生物技术通报,2010(3). |
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| 作者姓名： | 李喜艳 王加启 魏宏阳 卜登攀 胡菡 周凌云 |
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| 作者单位： | 中国农业科学院北京畜牧兽医研究所,动物营养学国家重点实验室,北京,100193 |
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| 摘 要： | 采用噻唑蓝比色法检测赖氨酸、蛋氨酸对体外培养的奶牛乳腺上皮细胞增殖的影响。赖氨酸和蛋氨酸在培养基中的添加浓度分别为0、0.05、0.2、0.4、0.8、1.6、3.2、6.4、12.8、25.6mmol/L和0、0.025、0.1、0.2、0.4、0.8、1.6、3.2、6.4、12.8mmol/L;培养期为24、48和72h。结果表明,赖氨酸在0.8-1.6mmol/L、蛋氨酸在0.4-0.8mmol/L浓度范围内对体外培养的奶牛乳腺上皮细胞增殖的促进作用最明显且在48h时增殖作用最强(P0.0001)。
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| 关 键 词： | 赖氨酸 蛋氨酸 奶牛乳腺上皮细胞 噻唑蓝比色法 |
The Effect of Lysine and Methionine on Proliferation of Bovine Mammary Epithelial Cells in vitro by MTT Colorimetric Assay |
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| Li Xiyan,Wang Jiaqi,Wei Hongyang,Bu Dengpan,Hu Han,Zhou Lingyun.The Effect of Lysine and Methionine on Proliferation of Bovine Mammary Epithelial Cells in vitro by MTT Colorimetric Assay[J].Biotechnology Bulletin,2010(3). |
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| Authors: | Li Xiyan Wang Jiaqi Wei Hongyang Bu Dengpan Hu Han Zhou Lingyun |
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| Institution: | Li Xiyan Wang Jiaqi Wei Hongyang Bu Dengpan Hu Han Zhou Lingyun(State Key Laboratory of Animal Nutrition,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193) |
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| Abstract: | Effects of lysine and methionine on proliferation of bovine mammary epithelial cells were evaluated by MTT colorimetric assay.The lysine was added to the DMEM/F12 culture medium at concentrations of 0,0.05,0.2,0.4,0.8,1.6,3.2,6.4,12.8,25.6 mmol/L and the methionine was added to the DMEM/F12 culture medium at concentrations of 0,0.025,0.1,0.2,0.4,0.8,1.6,3.2,6.4,12.8 mmol/L.Culture period was also considered,which was set as 24,48,72 h,respectively.The results showed that 0.8 to 1.6 mmol/L lysine and 0.4 to ... |
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| Keywords: | Lysine Methionine Bovine mammary epithelial cells MTT colorimetric assay |
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