Low Frequency Magnetic Fields Enhance Antitumor Immune Response against Mouse H22 Hepatocellular Carcinoma |
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| Authors: | Yunzhong Nie Yueqiu Chen Yongbin Mou Leihua Weng Zhenjun Xu Youwei Du Wenmei Wang Yayi Hou Tingting Wang |
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| Institution: | 1. State Key Laboratory of Pharmaceutical Biotechnology, Division of Immunology, Medical School, Nanjing University, Nanjing, China.; 2. Stomatological Hospital Affiliated Medical School, Nanjing University, Nanjing, China.; 3. National Laboratory of Solid Microstructures, Nanjing University, Nanjing, China.; 4. Jiangsu Key Laboratory of Molecular Medicine, Nanjing University, Nanjing, China.; Istituto Superiore di Sanità, Italy, |
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| Abstract: | ObjectiveMany studies have shown that magnetic fields (MF) inhibit tumor growth and influence the function of immune system. However, the effect of MF on mechanism of immunological function in tumor-bearing mice is still unclear.MethodsIn this study, tumor-bearing mice were prepared by subcutaneously inoculating Balb/c mice with hepatocarcinoma cell line H22. The mice were then exposed to a low frequency MF (0.4 T, 7.5 Hz) for 30 days. Survival rate, tumor growth and the innate and adaptive immune parameters were measured.ResultsMF treatment could prolong survival time (n = 28, p<0.05) and inhibit tumor growth (n = 9, p<0.01) in tumor-bearing mice. Moreover, this MF suppressed tumor-induced production of cytokines including interleukin-6 (IL-6), granulocyte colony- stimulating factor (G-CSF) and keratinocyte-derived chemokine (KC) (n = 9–10, p<0.05 or 0.01). Furthermore, MF exposure was associated with activation of macrophages and dendritic cells, enhanced profiles of CD4+ T and CD8+ T lymphocytes, the balance of Th17/Treg and reduced inhibitory function of Treg cells (n = 9–10, p<0.05 or 0.01) in the mice model.ConclusionThe inhibitory effect of MF on tumor growth was related to the improvement of immune function in the tumor-bearing mice. |
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