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A Simplified Positive-Sense-RNA Virus Construction Approach That Enhances Analysis Throughput |
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| Authors: | Bunpote Siridechadilok Methee Gomutsukhavadee Thunyarat Sawaengpol Sutha Sangiambut Chunya Puttikhunt Kwanrutai Chin-inmanu Prapat Suriyaphol Prida Malasit Gavin Screaton Juthathip Mongkolsapaya |
| Abstract: | Here we present an approach that advances the throughput of a genetic analysis of a positive-sense RNA virus by simplifying virus construction. It enabled comprehensive dissection of a complex, multigene phenotype through rapid derivation of a large number of chimeric viruses and construction of a mutant library directly from a virus pool. The versatility of the approach described here expands the applicability of diverse genetic approaches to study these viruses. |
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