Structure of the Bacterial Deacetylase LpxC Bound to the Nucleotide Reaction Product Reveals Mechanisms of Oxyanion Stabilization and Proton Transfer |
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| Authors: | Gina M Clayton Daniel J Klein Keith W Rickert Sangita B Patel Maria Kornienko Joan Zugay-Murphy John C Reid Srivanya Tummala Sujata Sharma Sheo B Singh Lynn Miesel Kevin J Lumb Stephen M Soisson |
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| Institution: | From ‡Global Structural Chemistry and ;§Screening and Protein Sciences, Merck Research Laboratories, West Point, Pennsylvania 19486 and ;¶Discovery Chemistry and ;‖Infectious Diseases, Merck Research Laboratories, Kenilworth, New Jersey 07033 |
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| Abstract: | The emergence of antibiotic-resistant strains of pathogenic bacteria is an increasing threat to global health that underscores an urgent need for an expanded antibacterial armamentarium. Gram-negative bacteria, such as Escherichia coli, have become increasingly important clinical pathogens with limited treatment options. This is due in part to their lipopolysaccharide (LPS) outer membrane components, which dually serve as endotoxins while also protecting Gram-negative bacteria from antibiotic entry. The LpxC enzyme catalyzes the committed step of LPS biosynthesis, making LpxC a promising target for new antibacterials. Here, we present the first structure of an LpxC enzyme in complex with the deacetylation reaction product, UDP-(3-O-(R-3-hydroxymyristoyl))-glucosamine. These studies provide valuable insight into recognition of substrates and products by LpxC and a platform for structure-guided drug discovery of broad spectrum Gram-negative antibiotics. |
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| Keywords: | Antibiotics Escherichia coli Lipopolysaccharide (LPS) Protein Structure X-ray Crystallography LpxC UDP |
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