Recent Technologies for Genetic Code Expansion and their Implications on Synthetic Biology Applications |
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| Affiliation: | 1. The Texas A&M Drug Discovery Laboratory, Department of Chemistry, Texas A&M University, College Station, TX 77843, USA;2. Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77843, USA;3. Institute of Biosciences and Technology and Department of Translational Medical Sciences, College of Medicine, Texas A&M University, Houston, TX 77030, USA;4. Department of Molecular and Cellular Medicine, College of Medicine, Texas A&M University, College Station, TX 77843, USA;1. Department of Chemistry, Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA;2. California Institute for Biomedical Research (Calibr), 11119 North Torrey Pines Road, La Jolla, CA 92307, USA;1. Department of Chemistry, Rice University, 6100 Main Street, Houston, TX 77005, United States;2. Department of Biosciences, Rice University, 6100 Main Street, Houston, TX 77005, United States;3. Department of Bioengineering, Rice University, 6100 Main Street, Houston, TX 77005, United States |
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| Abstract: | Genetic code expansion (GCE) enables the site-specific incorporation of non-canonical amino acids as novel building blocks for the investigation and manipulation of proteins. The advancement of genetic code expansion has been benefited from the development of synthetic biology, while genetic code expansion also helps to create more synthetic biology tools. In this review, we summarize recent advances in genetic code expansion brought by synthetic biology progresses, including engineering of the translation machinery, genome-wide codon reassignment, and the biosynthesis of non-canonical amino acids. We highlight the emerging application of this technology in construction of new synthetic biology parts, circuits, chassis, and products. |
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| Keywords: | non-canonical amino acid biosynthesis GCE optimization synthetic parts synthetic circuits synthetic chassis |
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