A method for the study of fungal growth inhibition by plant proteins |
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| Authors: | Alexander Ludwig Thomas Boller |
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| Institution: | Botanisches Institut der Universit?t Basel, Switzerland. |
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| Abstract: | A bioassay is described for the study of inhibitory activity of plant proteins on fungal growth. Fungal spores were germinated in liquid growth medium and pipetted into wells of a microtitre plate. Fungal growth was followed spectrophotometrically. The bioassay was tested using crude protein extracts from plant tissues known to have high activities of chitinase and beta-1,3-glucanase, and with purified enzymes. Crude protein preparations and combinations of the purified enzymes produced a temporary reduction of growth but no permanent growth inhibition. |
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| Keywords: | Growth inhibition assay Plant protein Fungal growth |
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