|
|||||
|
|
Effect of sucrose concentration on photosynthetic activity of in vitro cultures |
|
| Authors: | Jan J. Rybczyński Bożenna Borkowska Agnieszka Fiuk Helena Gawrońska Eleonora Śliwińska Anna Mikuła |
| Affiliation: | (1) Botanical Garden – Center for Biological Diversity Conservation, The Polish Academy of Sciences, Prawdziwka 2 str, 02-973 Warsaw, Poland;(2) Institute of Pomology and Floriculture, Pomologiczna 18 str, 96-100 Skierniewice, Poland;(3) Laboratory of Basic Sciences in Horticulture, Warsaw Agricultural University, Nowoursynowska 159, 02-776 Warsaw, Poland;(4) Department of Genetics and Plant Breeding, University of Technology and Agriculture, Kaliskiego 13 str, 85-789 Bydgoszcz, Poland |
| Abstract: | In this work, the effect of sucrose on photosynthetic activity during in vitro culture was studied. Experiments were carried out using uniform somatic embryo-derived germlings of Gentiana kurroo (Royle) confirmed by chromosome counting and flow cytometry technique. Photosynthetic activity was measured by chlorophyll a fluorescence and gas exchange method. The efficiency of photosynthetic apparatus as measured by the ratio F v/F m, Yield and qP (light phase of photosynthesis) was the highest when the medium was supplemented with 0.3% sucrose which well corresponded with plant gas exchange. Taking all data into consideration for the best development of photosynthetic apparatus and the most efficient of net photosynthesis of studied germlings would be medium supplemented with 0.2–0.4% of sucrose. |
| Keywords: | Chlorophyll a fluorescence Gas exchange Gentian Germlings Carbon source |
| 本文献已被 SpringerLink 等数据库收录！ | |