苹毛丽金龟对植物挥发物成分的触角电位及行为反应

为筛选适宜的苹毛丽金龟Proagopertha lucidula (Faldermann)引诱剂化学物质, 对已报道的金龟子科两性引诱剂单一组分进行了触角电位（EAG）和Y型嗅觉仪中的嗅觉反应测定。结果表明: 在10 μg刺激剂量下测试的11 种植物挥发物中, 只有顺-3-己烯-1-醇、香叶醇、茴香脑和丁香酚有明确的EAG反应值, 其他7种化合物具有弱反应值。剂量反应曲线表明, 具有明确的EAG活性的4种化合物激发的反应强度与化合物的剂量呈正相关。在Y型嗅觉仪中, 顺-3-己烯-1-醇、香叶醇、茴香脑和丁香酚4种化合物在1, 10, 100 μg剂量下的行为反应百分率都显著高于对照溶剂正己烷, 且雌雄之间无显著差异。该研究结果可为开发苹毛丽金龟田间引诱剂奠定基础。

Electroantennographic and behavioral responses of Proagopertha lucidula (Faldermann) (Coleoptera: Scarabaeidae) to plant volatiles

In order to screen attractive chemicals for trapping males and females Proagopertha lucidula (Faldermann), 11 plant volatiles that can trap male and female adults of other scarabs were used to test the electroantennogram (EAG) value and the taxis behavior of P. lucidula adults. The results showed that among the 11 test chemicals at the dosage of 10 μg, only (Z)-3-hexen-l-ol, geraniol, anethol and eugenol induced clear EAG response, while the other 7 chemicals elicited weak EAG response. The four compounds that induced clear EAG responses at the dose of 10 μg/μL elicited the dose-dependent EAG response. The taxis behavior of the beetle to the volatiles that induced clear EAG responses was also assayed at the doses of 1, 10 and 100 μg with Y-tube olfactometer. Corrected response percentages of both males and females to (Z)-3-hexen-l-ol, geraniol anethol and eugenol were significantly different from that of the hexane control. The study will provide the foundation for developing attractive chemicals for P. lucidula in fields.