Removal of estrogenic activity of iso-butylparaben and n-butylparaben by laccase in the presence of 1-hydroxybenzotriazole |
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Authors: | Hirohito Mizuno Hirofumi Hirai Shingo Kawai Tomoaki Nishida |
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Institution: | (1) Faculty of Agriculture, Shizuoka University, Ohya 836, Surugaku, Shizuoka 422-8529, Japan |
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Abstract: | In the presence of a redox mediator, 1-hydroxybenzotriazole (HBT), iso-butylparaben (iso-BP) and n-butylparaben (n-BP) were treated with laccase from white rot fungus Trametes versicolor. HPLC analysis demonstrated that iso-BP and n-BP almost completely disappeared from the reaction mixture after 4 h of treatment with the laccase-HBT system. Using the
yeast two-hybrid assay system, it was also confirmed that the laccase-HBT system substantially removed the estrogenic activity
of iso-BP and n-BP after 4 h of treatment. Furthermore, there was a linear relationship between the removal of estrogenic activity of both
parabens and the decrease in their concentrations. These results demonstrate that the laccase-HBT system is effective in eliminating
iso-BP and n-BP, and removing the estrogenic activity of both parabens. |
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Keywords: | iso-Butylparaben n-Butylparaben Estrogenic activity Laccase 1-Hydroxybenzotriazole White rot fungus |
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