Coamplification on chromosomes 7p12-13 and 9q12-13 identified by reverse chromosome painting in a glioblastoma multiforme |
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Authors: | Ulrike Fischer Bernd Wullich Hans-Peter Sattler Elisabeth Göttert Klaus D Zang Eckart Meese |
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Institution: | (1) Institut für Humangenetik, Universität des Saarlandes, D-66421 Homburg, Germany;(2) Abteilung für Urologie, Universität des Saarlandes, D-66421 Homburg, Germany;(3) Institut für Humangenetik, Universitätskliniken, Bau 68, D-66421 Homburg, Germany |
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Abstract: | DNA amplification is known to occur in approximately 50% of glioblastomas, with the epidermal growth factor receptor (EGFR) gene being the most frequently amplified. Whereas previous amplification studies have largely been limited to the analysis of known tumor-related genes, reverse chromosome painting allows us to search for as yet unidentified amplified domains. Here, we report the analysis of a glioblastoma multiforme by reverse chromosome painting. Hybridization signals were found on chromosome 7p12-13 and chromosome 9q12-13. Standard Southern blot analysis revealed amplification of the EGFR gene, which is localized on band 7p13. These findings corroborate previous reports on coamplification of sequences on different chromosomes in glioblastoma. |
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