A novel potassium channel encoded by Ectocarpus siliculosus virus |
| |
Authors: | Chen Jun Cassar Steven C Zhang Di Gopalakrishnan Murali |
| |
Institution: | Neuroscience Research, Global Pharmaceutical Research and Development, Abbott Laboratories, Abbott Park, IL 60064-6125, USA. jun.x.chen@abbott.com |
| |
Abstract: | Kcv, the first identified viral potassium channel encoded by the green algae Paramecium bursaria chlorella virus (PBCV-1), conducted K(+) selective currents when expressed in heterologous systems. This K(+) channel was proposed to be important for PBCV-1 infection and replication. In the present study, we identified and functionally characterized a novel K(+) channel Kesv, encoded by Ectocarpus siliculosus virus that infects filamentous marine brown algae. Kesv encodes a protein of 124 amino acids and is 21.8% identical and 37.1% homologous to Kcv. Membrane topology programs predicted that Kesv consists of three transmembrane domains. When expressed in Xenopus oocytes, Kesv induced largely instantaneous, K(+) selective currents that were sensitive to block by Ba(2+) and amantadine. Thus, Kesv along with Kcv, constitutes an emerging family of viral potassium channels, which may play important roles in the life cycle of viruses. |
| |
Keywords: | Potassium channel Ectocarpus siliculosus virus Paramecium bursaria chlorella virus Viral life cycle |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|