| 不同长度poly(C)对口蹄疫病毒基因工程毒的毒力影响 |
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| 引用本文： | 白兴文,李平花,孙普,李永亮,包慧芳,卢曾军,曹轶梅,郭建宏,刘在新. 不同长度poly(C)对口蹄疫病毒基因工程毒的毒力影响[J]. 微生物学报, 2008, 48(12): 1654-1658 |
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| 作者姓名： | 白兴文 李平花 孙普 李永亮 包慧芳 卢曾军 曹轶梅 郭建宏 刘在新 |
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| 作者单位： | 中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室,国家口蹄疫参考实验室,农业部畜禽病毒学重点开放实验室,兰州,730046 |
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| 基金项目： | 国家“973项目”(2005CB23201); “十一五”国家科技支撑计划项目(2006BAD06A03) |
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| 摘 要： | 【目的】研究口蹄疫病毒(foot-and-mouth disease virus，FMDV) poly(C)区段的序列长度与FMDV毒力之间的关系。【方法】首先利用NheⅠ/NotⅠ线化FMDV pGEM-XJ/AKT/69全长重组质粒，制备体外转录本，借助Lipofectamine 2000转染BHK-21细胞，获得基因工程毒。随后，在细胞传代过程中，收取不同代数病毒培养液，提取总RNA进行poly(C)的长度测定，并进行乳鼠致病性试验和BHK-21细胞TCID50的测定。【结果】我们发现，基因工程毒在BHK-21上连续传代至第6代时，可见明显的致细胞病变效应(cytopathic effect，CPE)；在经过一代乳鼠接种之后再重新适应到BHK-21细胞的过程中，poly(C)区段出现了缩短现象；乳鼠致病性试验和BHK-21细胞TCID50测定结果表明，尽管基因工程毒与母本毒相比毒力偏低，但含有不同长度poly(C)的基因工程毒之间并无显著差异。【结论】poly(C)区段的序列长度在12~17个C之间改变对FMDV基因工程毒的毒力无明显的影响。
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| 关 键 词： | 口蹄疫病毒；基因工程毒；poly(C)；毒力 |
| 收稿时间： | 2008-05-13 |
| 修稿时间： | 2008-07-19 |
Virulence comparison of genetic engineering virus containing different length poly(C) tract of foot-and-mouth disease virus |
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| Xingwen Bai,Pinghua Li,Pu Sun,Yongliang Li,Huifang Bao,Zengjun Lu,Yimei Cao,Jianhong Guo and Zaixin Liu. Virulence comparison of genetic engineering virus containing different length poly(C) tract of foot-and-mouth disease virus[J]. Acta microbiologica Sinica, 2008, 48(12): 1654-1658 |
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| Authors: | Xingwen Bai Pinghua Li Pu Sun Yongliang Li Huifang Bao Zengjun Lu Yimei Cao Jianhong Guo Zaixin Liu |
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| Affiliation: | Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Sciences, State Key Laboratory of Veterinary Etiological biology, National Foot-and-Mouth Disease Reference Laboratory, Key Laboratoryof Animal Virology of Ministry of Agriculture, L;Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Sciences, State Key Laboratory of Veterinary Etiological biology, National Foot-and-Mouth Disease Reference Laboratory, Key Laboratoryof Animal Virology of Ministry of Agriculture, L;Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Sciences, State Key Laboratory of Veterinary Etiological biology, National Foot-and-Mouth Disease Reference Laboratory, Key Laboratoryof Animal Virology of Ministry of Agriculture, L;Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Sciences, State Key Laboratory of Veterinary Etiological biology, National Foot-and-Mouth Disease Reference Laboratory, Key Laboratoryof Animal Virology of Ministry of Agriculture, L;Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Sciences, State Key Laboratory of Veterinary Etiological biology, National Foot-and-Mouth Disease Reference Laboratory, Key Laboratoryof Animal Virology of Ministry of Agriculture, L;Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Sciences, State Key Laboratory of Veterinary Etiological biology, National Foot-and-Mouth Disease Reference Laboratory, Key Laboratoryof Animal Virology of Ministry of Agriculture, L;Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Sciences, State Key Laboratory of Veterinary Etiological biology, National Foot-and-Mouth Disease Reference Laboratory, Key Laboratoryof Animal Virology of Ministry of Agriculture, L;Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Sciences, State Key Laboratory of Veterinary Etiological biology, National Foot-and-Mouth Disease Reference Laboratory, Key Laboratoryof Animal Virology of Ministry of Agriculture, L;Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Sciences, State Key Laboratory of Veterinary Etiological biology, National Foot-and-Mouth Disease Reference Laboratory, Key Laboratoryof Animal Virology of Ministry of Agriculture, L |
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| Abstract: | |
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| Keywords: | foot-and-mouth disease virus genetic engineering virus poly(C) virulence |
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