Silk sericin protein of tropical tasar silkworm inhibits UVB-induced apoptosis in human skin keratinocytes |
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Authors: | Rupesh Dash Mahitosh Mandal Sudip K Ghosh S C Kundu |
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Institution: | (1) Department of Biotechnology, Indian Institute of Technology, Kharagpur, 721302, India;(2) School of Medical Science and Technology, Indian Institute of Technology, Kharagpur, India |
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Abstract: | The silk protein sericin has been identified as a potent antioxidant and photoprotective agent against ultraviolet B (UVB)
irradiation in mouse skin model. In this study, we have investigated the anti-apoptotic effect of sericin in UVB (30 mJ/cm2)-irradiated human keratinocytes. Flow cytometry analysis has shown that pre-treatment with sericin inhibits UVB-induced apoptosis.
The pre-treatment with sericin suppresses bax expression, up-regulates the expression of bcl-2, prevents both the activation
of caspase-3 and cleavage of Poly (ADP-ribose) polymerase. Generation of intracellular hydrogen peroxide in UVB-treated keratinocytes
is inhibited through pre-treatment with sericin suggesting that sericin probably prevents mitochondrial damage.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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Keywords: | Sericin Keratinocytes UVB H2O2 |
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