Cloning and characterization of a gene coding for a major extracellular chitosanase from the koji mold Aspergillus oryzae |
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| Authors: | Sugita Akihiro Sugii Ayako Sato Kazuaki Zhang Xiao-Yong Dai An-Lan Taguchi Goro Shimosaka Makoto |
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| Affiliation: | Division of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano, Japan. |
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| Abstract: | A gene coding for a major extracellular chitosanase was isolated from Aspergillus oryzae IAM2660. It had a multi-domain structure composed of a signal peptide, a catalytic domain, Thr- and Pro-rich linkers, and repeated peptides (the R3 domain) from the N-terminus. The R3 domain bound to insoluble powder chitosan, but it did not promote the hydrolysis rate of the chitosanase to any extent. |
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