Active suppression of EndoPG IV by ligation of the pro-sequence from Stereum purpureum Pro-EndoPG I |
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Authors: | Kimura Tsubasa Ogawa Shun Matsuda Motoki Shimizu Tetsuya Miyairi Kazuo |
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Institution: | Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, Aomori, Japan. |
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Abstract: | We attempted to inactivate endopolygaolacturonase from Stereum purpureum (EndoPG) IV of identical origin by linking the pro-sequence of S. purpureum Pro-EndoPG I to the C-terminus. The recombinant Pro-EndoPG IV, expressed in Escherichia coli, had no polygalacturonase (PG) activity, but activity was acquired after partial degradation of the pro-sequence with V8 protease, as was the case for Pro-EndoPG I. These results indicate that the pro-sequence of Pro-EndoPG I can suppress the PG activity of EndoPG IV. |
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