Sequential insertion of multiple I-SceI recognition sites at designed loci of the Bacillus subtilis 168 genome |
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Authors: | Itaya Mitsuhiro |
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Affiliation: | Mitsubishi Kagaku Institute of Life Sciences, Machida, Tokyo, Japan. mita2001@sfc.keio.ac.jp |
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Abstract: | Bacillus subtilis 168 is the only bacterium-based host serving for the cloning of giant DNA above 1.000 kbp. As rapid verification of the genome structure is crucial during the cloning process, six of 18-base sequence recognized by endonuclease I-SceI were sequentially created in the B. subtilis 168 genome. The established method and materials should be of use for other B. subtilis derivatives. |
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