Genetic mapping of a new semi-dwarf gene, sd-t(t), in indica rice and estimating of the physical distance of the mapping region |
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Authors: | JIANG Guanghuai LIANG Guohua ZHAI Wenxue GU Minghong LU Runlong XU Jichen ZHU Lihuang |
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Institution: | School of Life Science, University of Science and Technology of China, 230026, Hefei, China. |
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Abstract: | Application and functional study of dwarf and semi-dwarf genes are of great importance to both crop breeding and molecular biology. A new semi-dwarf gene, sd-t(t), non-allelic to sd-1, had been identified in an indica rice variety, Aitaiyin 2. In this study the gene was genetically mapped by using an F2 population, which consisted of 474 individuals developed from a cross between Aitaiyin 2 and B30. The sd-t(t) gene was located between the RFLP markers R514 and R1408B with a distance of 1.1 cM to R514, and 4.5 cM to R1408B on chromosome 4. A physical contig covering the sd-t(t) mapping region was further constructed by screening a BAC library with R514 and R1408B as probes, and the physical distance between R514 and R1408B was estimated at approximately 147 kb. This result will facilitate map-based cloning of the sd-t(t) gene. |
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Keywords: | semi-dwarf gene sd-t(t) simple sequence length polymorphism (SSLP) restriction fragment length polymorphism (RFLP) molecular marker genetic mapping BAC contig |
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