Absence of mouse mammary tumor virus proviral amplification in chemically induced lymphomas of RF/J mice. |
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Authors: | R Kraft S T Ishizaka S A Okenquist G Childs F Lilly J Lenz |
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Affiliation: | Department of Molecular Genetics, Albert Einstein College of Medicine, Bronx, New York 10461. |
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Abstract: | RF/J mice are susceptible to the induction of thymic lymphomas by the carcinogens 3-methylcholanthrene and N-methyl-N-nitrosourea. Given the association of mouse mammary tumor virus (MMTV) with certain thymomas, we examined genomic DNA from chemically induced lymphomas of RF/J mice for new MMTV proviruses. Of 13 tissue culture lines derived from 3-methylcholanthrene-induced tumors, 5 had acquired new proviruses. MMTV amplification coincided with the appearance of viral mRNAs and proteins. However, no primary tumors or animal-passaged tumors contained new proviruses. These observations indicate that MMTV does not have a role in the tumor induction process, although it may become activated and amplified in tissue culture lines derived from tumors. |
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