Application of photon correlation spectroscopy as a technique for detecting culture contamination |
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Authors: | Jepras R I Perkins E A Rarity J Carr R J Clarke D J Atkinson T |
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Institution: | Sensor Group, Division of Biotechnology, PHLS Centre for Applied Microbiology and Research, Porton Down, Wilshire SP4 0JG, United Kingdom. |
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Abstract: | The application of photon correlation spectroscopy (PCS) to detect culture contamination in chemostats was studied. It was found that the presence of a given particle size in a population of particles of a different size could be detected, but this ability was strongly dependent on particles of a different size could be detected, but this ability was strongly dependent on particle size difference and was most sensitive when contaminants are larger than the host. The inherent polydisparity of actively growing and dividing microbial cells negates any advantage in the use of multi-angle PCS to detect contaminants. |
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Keywords: | culture contamination photon correlation spectroscopy dynamic light scattering particle sizing mixed culture |
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