珍稀濒危植物金毛狗的SRAP分析

利用SRAP分子标记技术,对7个居群79株金毛狗进行遗传多样性分析。结果表明:10对SRAP引物组合共得到107条扩增条带,多态性条带比率为85.98%,Nei’s基因多样性指数为0.229 6,Shannon’s多样性指数为0.358 6,表明金毛狗居群水平具有较高的遗传多样性;金毛狗7个居群的总基因多样度为0.229 6,居群内遗传多样度为0.135 4,居群间的遗传分化指数为0.410 6,表明有41.06%的变异存在于居群间,有58.94%的变异存在于居群内;居群间基因流为0.717 8,表明居群间基因交流频率较低;遗传一致度和UPGMA聚类分析结果显示,生境条件相似的居群优先聚集,说明金毛狗种质亲缘关系与地理分布相关性不显著。

Analysis of Genetic Diversity of the Rare and Endangered Species Cibotium barometz by SRAP Markers

SRAP molecular markers were used to study the genetic diversity on population and individual levels of 79 Cibotium barometz which were from 7 populations.The results showed that:10 SRAP primer combination amplified 107 bands with 91(85.98%) polymorphic.Nei’s gene diversity index of SRAP was 0.229 6.Shannon’s information index was 0.358 6.That implied that there was high genetic diversity among the popolations.The total genetic diversity was 0.229 6.The genetic diversity within population and the coefficient of gene differentiation were 0.135 4 and 0.410 6,respectively.That implied that it was 41.06% of genetic diversity among populations,and was 58.94% of genetic diversity within populations.Gene flow among population was 0.717 8 which implied that the gene flow was at a low level.The genetic similarity coefficient and the UPGMA dendrogram indicated that populations with similar conditions were clustered firstly,the geographical distribution was not distinctive.