紫斑牡丹遗传多样性的ISSR分析

利用ISSR标记对一个居群内的16个紫斑牡丹品种材料进行基因组多态性分析,从100条引物中筛选出15条用于紫斑牡丹的ISSR扩增。共扩增出134条带,其中多态性条带96条,多态性百分率为71.6%。根据ISSR扩增结果,利用NTSYSpc 2.10e软件进行Jaccard相似性系数分析,16个紫斑牡丹品种的遗传相似系数为0.45～0.93。UPGMA聚类分析结果显示,在遗传相似系数0.534处,16个紫斑牡丹品种材料可分为4类。第Ⅰ类中的6个紫斑牡丹品种材料中有5个为皇冠型品种,1个单瓣型,其他类中也有皇冠花型品种但未聚在Ⅰ类中;第Ⅱ类仅有1个‘粉盘托桂’,它是所选材料中唯一的托桂型品种,单独聚为一类;第Ⅲ类由不同花色、花型品种聚为一类,可见亲缘关系较近的紫斑牡丹品种性状变异性也很大;第Ⅳ类中A组品种材料均为白色,花型有单瓣型、荷花型和绣球型;B组仅有一个‘银线女’,它是所选材料中唯一的红色绣球型材料。研究表明,不同相似系数的遗传聚类划分与花色、花型之间并非完全具有相关性。

Genetic Diversity of Paeonia rockii Revealed by ISSRs

The genetic diversity of 16 Paeonia rockii lines in one population was analyzed by using inter-simple sequence repeat markers(ISSR).Fifteen primers selected from 100 primers were used for ISSR amplification.A total of 134 bands were generated,of which 96 bands were polymorphic bands(the percentage of polymorphic band,PPB=71.6%).According to the results of ISSR amplification,which were analyzed to Jaccard similarity coefficient by NTSYSpc 2.10e software,the genetic similarity coefficient varied from 0.45 to 0.93.The clustering dendrogram was constructed by UPGMA method.Sixteen P.rockii materials were divided into four major groups while the similarity coefficient was 0.534.Class Ⅰ included six of P.rockii materials,and five in six was crown form,left a single form species.But other classes also have crown flower varieties.Class Ⅱ had an anemone form species,it was the only anemone species elected in the selected materials.Four different color and form materials were clustered into Class Ⅲ,suggest that the character of closer phylogenetic relationship species were varieties.A group of Class Ⅳ included four white flowers materials,whose form were different.Group B was P.rockii ’Yinxiannv’ alone,it was the only red material selected.Thus,genetic clustering classification of different resemble coefficients was not consisted with that based on the flower color and form.