Catecholaminergic innervation of pyramidal and GABAergic nonpyramidal neurons in the rat hippocampus |
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Authors: | M. Frotscher C. Leranth |
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Affiliation: | (1) Institute of Anatomy, Johann Wolfgang Goethe University, Theodor-Stern-Kai 7, D-6000 Frankfurt am Main 70, Federal Republic of Germany;(2) Section of Neuroanatomy, Yale University School of Medicine, 333 Cedar Street, P.O. Box 3333, 06510 New Haven, CT, USA |
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Abstract: | Summary This study describes the catecholaminergic innervation of rat hippocampal neurons at the electron microscopic level by using an antibody against tyrosine hydroxylase (TH) and immunocytochemical techniques. In a first series of experiments, the course and distribution as well as the synaptic contacts of TH-immunoreactive fibers were analyzed with the peroxidase-antiperoxidase (PAP) method. Next, peroxidase immunostaining of TH fibers was combined with glutamate decarboxylase (GAD) immunostaining, using avidinated ferritin as a second electrondense marker. Our results demonstrate that TH-immunostained terminals establish asymmetric synaptic contacts with spines of pyramidal neurons, and symmetric synaptic contacts with cell bodies and dendritic shafts of ferritin-labeled GAD-immunoreactive nonpyramidal cells.Dedicated to Professor Dr. T.H. Schiebler on the occasion of his 65th birthday. |
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