HPV-16、EB病毒对细胞增殖的影响及与子宫颈癌发病的关系

目的 了解宫颈癌患者人乳头瘤病毒16（HPV 16）、EB病毒（EBV）感染情况，探讨HPV-16、EBV对细胞核增殖性抗原（PCNA）表达的影响及在子宫颈癌发病中的意义。方法 免疫组织化学SP法检测59例宫颈癌和20例非癌性子宫颈上皮细胞中HPV-16、EBV蛋白表达和PCNA表达的情况，并分析它们的表达与病理参数的关系。结果 子宫颈癌和非癌性子宫颈上皮细胞中HPV-16、EBV及PCNA的阳性表达率依次分别为69．49％、57．63％及77．97％和30％、25％及15％；子宫颈癌和非癌性子宫颈上皮细胞中HPV-16、EBV的共同阳性表达率分别为35．59％和0％，子宫颈癌中HPV-16、EBV及PCNA的阳性表达率均高于非癌性子宫颈上皮（P〈0．05）。病理Ⅰ级、Ⅱ级和Ⅲ级子宫颈癌中HPV-16、EBV、PCNA的阳性表达率分别为65．00％、55．00％、60．00％，69．57％、60．87％、82．61％和75．OO％、56．25％、93．75％。各级子宫颈癌间HPV-16、EBV的阳性表达率差异无显著性，但PCNA的表达率随病理分级的增加显著上升（P〈0．05）。不同期别子宫颈癌问HPV-16、EBV及PCNA的阳性表达率差异无显著性。HPV-16阳性与阴性组子宫颈上皮PCNA的阳性表达率分别为82．98％（39／47）与43．75％（14／32），两者差异有显著性（P〈0．05）。EBV阳性及阴性组子宫颈上皮PCNA的阳性表达率分别为71．79％（28／39）及42．50％（17／40），两者差异有显著性（P〈0．05）。HPV-16和EBV共同阳性表达的21例子宫颈癌PCNA阳性表达率均为100％。结论 HPV-16、EBV通过增加PCNA表达的促细胞增生作用可能是子宫颈癌的发生机制之一。

HPV-16 and EBV infections associating with expression of PCNA in the carcinogenesis of cervical carcinoma

Objective To illustrate the influences of HPV-16 and EBV to proliferate cellular nuclear antigen (PCNA) and to evaluate their role in the occurrence of cervical carcinoma. Methods Formalin-fixed and paraffin-embedded tissues from 59 cases of cervical carcinoma and 20 cases of cervical non-carcinoma were used to detect protein expressions of HPV-16, EBV and PCNA by immunohistochemistry method. Results The expression rates of HPV-16, EBV and PCNA were 69.49%, 57.63% and 77.97% in the tissues from cervical carcinoma and 30%, 25% and 15% in the tissues from cervical non-carcinoma respectively. The coexisted rates of HPV-16 and EBV were 35.59% in those from cervical carcinoma, while 0% from cervical non-carcinoma. The expression rates of HPV-16, EBV and PCNA in cervical carcinoma were all higher than those in cervical non-carcinoma. The expression rates of HPV-16 and EBV were not significantly different among tissues from cervical carcinoma in different grades and phases. PCNA expression rate increased with the grades of the carcinoma, while it did not with different phases. The PCNA expression rate was higher in the tissues with HPV-16 or EBV infection than that without. The expression rate of PCNA was 100% in the tissues infected with HPV-16 and EBV simultaneously. Conclusions The infection of HPV-16 and EBV may contribute to carcinogenesis of cervical carcinoma by increasing PCNA expression. The expression of PCNA may be served as an prediction of carcinogenesis.