军曹鱼淋巴囊肿病毒主衣壳蛋白基因全序列分析

军曹鱼(Rachycentron canadum)亦称海鲡,是我国南方沿海一带的重要海水网箱养殖对象。2005年8月,广东省海水网箱养殖的军曹鱼首次暴发类似的淋巴囊肿病,病鱼的口唇、鳃、鳍、尾及体表等处,可看到大小不一的单个或成群的肿瘤,个别网箱的感染率在80%以上,死亡率近30%。病鱼形象丑陋,严重影响其市场价值,造成了较大的经济损失。淋巴囊肿病(Lymphocystis disease)发现于1874年,1965年正式确认该病病原为淋巴囊肿病毒1],现已知可感染9目34科140种以上鱼类。我国在20世纪90年代陆续在养殖石斑鱼、鲈鱼、牙鲆中发现淋巴囊肿病2-5],随后对其病原…

Inverse PCR Amplification of the Complete Major Capsid Protein Gene of Lymphocystis Disease Virus Isolated From Rachycentron canadum and the Phylogenetic Analysis of the Virus

The major capsid protein of lymphocystis disease virus isolated from Rachycentron canadum(LCDV-rc) was amplified and analysed.The 457bp DNA core fragment was amplified with the degenerate primers designed according to the conserved sequences of MCP gene of iridoviruses,then the flaking sequences adjacent to the core region were amplified by inverse PCR,and the complete sequence was obtained by combining all of them.The open reading frame of the gene is 1380bp in length,encoding a putative protein of 459 aa with molecular weight 51.12 kD and pI 6.87.Constructing the phylogenetic tree for comparing the MCP amino acid of iridoviruses,the results indicated that LCDV-rc is most homologous to the other Lymphocystis viruses and all of them constitute a branch.Accordingly LCDV-rc is identified as Lymphocystivirus.