Identification and Semi-Quantification of Gibberellins from the Pollen and Anthers of Zea mays by Immunoassay and GC/MS |
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Authors: | Yamaguchi Isomaro; Nakazawa Hideyuki; Nakagawa Ryusuke; Suzuki Yoshihito; Kurogochi Shin; Murofushi Noboru; Takahashi Nobutaka; Weiler Elmar W |
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Institution: | 1Department of Agricultural Chemistry, The University of Tokyo Bunkyo-ku, Tokyo, 113 Japan
2Nihon Tokushu-Noyaku Co. Ltd. Yuki-Shi, Ibaraki-ken, 307 Japan
3Department of Plant Physiology, Ruhr-University Bochum D 4630 Bochum 1, Federal Republic of Germany |
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Abstract: | Radioimmunoassays and enzyme-linked immunosorbent assays formethyl esters of gibberellins A1, A3, A4, and A7 were establishedusing an antiserum specific for GA1-Me. The antiserum was characterizedby high titer and specificity for such C19-GAs with 3ß-hydroxylgroup as GA1, GA3, GA4 and GA7. Combination of this antiserumand HPLC enabled us to identify and quantify GA, and GA4 fromthe pollen of Zea mays with a high degree of reliability. Similarly,identification and quantification of GA9 and GA20 were alsomade possible by use of an antiserum specific for GA20-Me. Combineduse of immunoassays and GC/MS enabled us to identify nine GAsfrom the pollen and four from the anthers of Zea mays. The identificationof non-13-hydroxylated GAs, such as GA4 and GA9, in additionto 13-hydroxylated GAs from the pollen and the anthers suggeststhat the early-non-hydroxylation pathway, as well as the early-13-hydrox-ylationpathway, operates in the male reproductive organs of Zea mays,and that the organ-specific biosynthesis and/or localizationof GAs in Zea mays is similar to that in Oryza saliva. (Received May 7, 1990; Accepted August 20, 1990) |
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