| 靶向survivin基因的siRNA对姜黄素诱导胃癌细胞凋亡的影响 |
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| 引用本文： | 谭曙光,屈顺林.靶向survivin基因的siRNA对姜黄素诱导胃癌细胞凋亡的影响[J].现代生物医学进展,2010,10(3). |
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| 作者姓名： | 谭曙光 屈顺林 |
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| 作者单位： | 1. 衡阳市中心医院肿瘤外科,湖南,衡阳,421001
2. 南华大学医学院病生教研室,湖南,衡阳,421001 |
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| 基金项目： | 湖南省卫生厅科研基金(B2006-101) |
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| 摘 要： | 目的:研究靶向survivin基因的siRNA对胃癌细胞,survivin表达的影响,抑制survivin基因表达对姜黄素诱导胃癌细胞凋亡的影响。方法:通过脂质体将survivinsiRNA导入胃癌细胞株BGC-803,用Real-timePCR和Western-blotting检测转染后细胞内survivin基因表达水平,流式细胞仪和Hochest染色检测细胞凋亡的改变。结果:姜黄素可抑制BGC-803细胞的生长,其生长抑制率和药物浓度与作用时间呈依赖关系;姜黄素作用BGC-803细胞后,survivin蛋白和mRNA表达降低;通过转染survivinsiRNA抑制BGC-803细胞survivin基因的表达能促进姜黄素诱导BGC-803细胞凋亡的作用。结论:靶向抑制survivin基因表达后姜黄素诱导胃癌细胞BGC-803凋亡的作用增强。
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| 关 键 词： | 姜黄素 生存素 凋亡 胃癌细胞 |
Effect of Survivin siRNA on Gastric Cancer Cell Apoptosis Induced by Curcumin |
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| TAN Shu-guang.Effect of Survivin siRNA on Gastric Cancer Cell Apoptosis Induced by Curcumin[J].Progress in Modern Biomedicine,2010,10(3). |
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| Authors: | TAN Shu-guang |
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| Institution: | TAN Shu-guang (Deptment of Tumor Surgery,Hengyang Central Hospital,Hengyang,421001,Hunan China) |
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| Abstract: | Objective:This study was to investigate the effect of survivin siRNA on survivin expression and gastric cancer cell apoptosis induced by curcumin. Methods:Survivin siRNA was transfected into BGC-803 cells to inhibit the expression of survivin. mRNA and protein levels of survivin were detected by real-time polymerase chain reaction (RT-PCR) and Western Blotting. Cell apoptosis was assayed by flow cytometry (FCM) and Hochest staining. Results:Curcumin could effective inhibit the growh of the BGC-803 cells in ... |
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| Keywords: | Curcumin Survivin Apoptosis Gastric cancer |
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