Liquid chromatography tandem mass spectrometry method for simultaneous determination of metoprolol tartrate and ramipril in human plasma |
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Authors: | Gowda K Veeran Mandal Uttam Senthamil Selvan P Sam Solomon W D Ghosh Animesh Sarkar Amlan Kanti Agarwal Sangita Nageswar Rao T Pal Tapan Kumar |
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Affiliation: | Bioequivalence Study Centre, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, India. mailveeran@yahoo.com |
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Abstract: | A simple, rapid, sensitive and specific liquid chromatography-tandem mass spectrometry method was developed and validated for quantification of metoprolol tartrate (MT) and ramipril, in human plasma. Both the drugs were extracted by liquid-liquid extraction with diethyl ether-dichloromethane (70:30, v/v). The chromatographic separation was performed on a reversed-phase C8 column with a mobile phase of 10 mM ammonium formate-methanol (3:97, v/v). The protonated analyte was quantitated in positive ionization by multiple reaction monitoring with a mass spectrometer. The method was validated over the concentration range of 5-500 ng/ml for metoprolol and ramipril in human plasma. The precursor to product ion transitions of m/z 268.0-103.10 and m/z 417.20-117.20 were used to measure metoprolol and ramipril, respectively. |
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