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九孔鲍四倍体诱导的研究
引用本文:严正凛.九孔鲍四倍体诱导的研究[J].生物技术通报,2008(Z1).
作者姓名:严正凛
作者单位:集美大学水产学院省高校水产科学技术与食品安全重点实验室,厦门,361021
基金项目:集美大学科研基金资助;;福建省科技计划重大项目(编号2002N003)资助
摘    要:采用二倍体鲍卵子,抑制其第一极体、抑制其第一极体和第二极体诱导四倍体,用三倍体鲍卵子与普通鲍精子受精并抑制其第一极体,以及用三倍体鲍的其它方法诱导四倍体。用倍体分析仪检测DNA相对含量以判别其倍性。实验结果,先后共培育出存活的四倍体鲍239个,个体大小为2.5~6.0cm;挑选性腺发育较好的四倍体鲍(包括雌雄),与普通鲍杂交繁育三倍体,先后共培育出10批次,培育出的苗量共计15.8万个。

关 键 词:九孔鲍  三倍体  四倍体  诱导  育种  

Studies on Induction of Tetraploid Abalone Haliotis diversicolor aquatilis
Yan Zhenglin.Studies on Induction of Tetraploid Abalone Haliotis diversicolor aquatilis[J].Biotechnology Bulletin,2008(Z1).
Authors:Yan Zhenglin
Institution:The Key Laboratory of Science and Technology for Aquaculture and Food Safety;Fisheries College;Jimei Univeristy;Xiamen 361021
Abstract:Tetraploid abalones Haliotis diversicolor aquatilis were induced by inhibiting releasing the first polar body, the first and second polar bodies of diploid eggs,and by inhibiting the release of the first polar body of zygotes fertilized between ordinary sperms and triploid eggs,as well as other methods of triploid were used to induce tetraploid.The relative DNA content was measured by using the Ploidy Analyser to identify the ploidy of induced abalones.ResultS showed that 239 survival tetraploid abalones we...
Keywords:Haliotis diversicolor aquatilis  Triploid  Tetraploid  Induction  Seed breeding  
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