一株产漆酶真菌新月弯孢霉JQH-100在染料脱色中的应用

从感染叶斑病的玉米叶片中分离、纯化得到一株高产漆酶的新月弯孢霉Curvularia lunata JQH-100菌株。液体培养Curvularia lunata JQH-100可产漆酶且活性较高,产酶高峰出现在第3天;以ABTS为底物粗酶液的最适反应温度是30℃,最适反应pH是2.8;染料脱色的研究表明,共培养体系对茜素红的脱色率达到了92.6%,对中性红和刚果红的脱色率也都在80%以上;Curvularia lunata JQH-100所产漆酶经纯化后对染料茜素红和刚果红有较高的脱色率,分别为82.1%和81.2%。研究结果显示Curvularia lunata JQH-100在染料废水处理中有较大应用潜力。

Application of a laccase-producing fungus strain Curvularia lunata JQH-100 to dye decolorization

A fungal strain Curvularia lunata JQH-100 with high laccase-producing ability was isolated from maize leaves infected by curvularia disease. The highest laccase activity was found in the third day when Curvularia lunata JQH-100 was cultivated in the liquid medium. The optimum temperature and pH of crude enzyme using 2,2′-Azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) as the substrate were 30℃ and 2.8, respectively. Several synthetic dyes were added into the culture medium to test the decolorization ability of Curvularia lunata. The results showed that the co-culture system decolorized 92.6% of Alizarin Red and more than 80% of Neutral and Congo Red. Laccase from Curvularia lunata was also purified for further assay of decolorizing Alizarin and Congo Red, which resulted in a decolorization percentage of 82.1% for Alizarin Red and 81.2% for Congo Red. This indicates that Curvularia lunata JQH-100 may be applied for treating dye-contaminated wastewater.