Lymphatic endothelial celline (CH3) from a recurrent retroperitoneal lymphangioma |
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Authors: | Dennis Way Mary Hendrix Marlys Witte Charles Witte Ray Nagle John Davis |
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Institution: | (1) Departments of Pathology, Anatomy, and Surgery, University of Arizona College of Medicine, 85724 Tucson, Arizona |
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Abstract: | Summary An endothelial cell line derived from a massive recurrent chyle-containing retroperitoneal lymphangioma was isolated in monolayer
culture. Scanning and transmission electron microscopy and immunohistochemistry confirmed a close resemblance to blood vascular
endothelium with typical cobblestone morphology, positive immunofluorescence staining for endothelial marker Factor VIII-associated
antigen and fibronectin, and prominent Weibel-Palade bodies. The endothelial cells also exhibited other ultrastructural features
characteristic of lymphatic endothelium, including sparse microvillous surface projections, overlapping intercellular junctions,
and abundant intermediate filaments. This endothelial cell line represents a new source of proliferating lymphatic endothelium
for future study, including structural and functional comparison to blood vascular endothelium.
Supported in part by Arizona Disease Control Research Commission contracts 8277-000000-1-1-AT-6625 and ZB-7492. Presented
in part at the 10th International Congress of Lymphology in Adelaide, Australia, August 1985. |
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Keywords: | lymphatics endothelium lymphangioma |
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