The mechanism of hatching of eggs of Haemonchus contortus |
| |
Authors: | W P Rogers F Brooks |
| |
Institution: | Department of Entomology, Waite Agricultural Research Institute, Glen Osmond, S.A. 5064 U.S.A |
| |
Abstract: | Fluid collected from hatching eggs of Haemonchus contortus contained a lipase which hydrolysed 2-naphthyl laurate (about 0·7 μmol naphthol freed /h/106 eggs). The fluid also hydrolysed l-leucinamide (about 2·3 μmol leucine freed/h/106 eggs). The fluid when added to normal or heated eggs caused ‘hatching’. ‘Hatching’ also occurred in exsheathing fluid from infective juveniles and in a preparation of pancreatic lipase containing leucine aminopeptidase. A purified mammalian leucine aminopeptidase in combination with several different lipases did not attack egg shells.The ‘spontaneous’ hatching of eggs of H. contortus was strongly inhibited by 1,10-phenanthroline, 10?3M, and this inhibition was reversed by Zn2+. However, the inhibition of ‘hatching’ of eggs in externally applied hatching fluid, or the hydrolysis of leucinamide in hatching fluid was generally less marked. |
| |
Keywords: | eggs hatching lipase leucine aminopeptidase |
本文献已被 ScienceDirect 等数据库收录! |
|