| 高糖对人肾小球系膜细胞SREBP-1、FAS表达的影响 |
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| 引用本文： | 郝军,朱琳,赵松,刘巍,要红叶,刘淑霞,段惠军.高糖对人肾小球系膜细胞SREBP-1、FAS表达的影响[J].中国组织化学与细胞化学杂志,2009,18(2):156-160. |
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| 作者姓名： | 郝军 朱琳 赵松 刘巍 要红叶 刘淑霞 段惠军 |
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| 作者单位： | 1. 河北医科大学病理学教研室,石家庄,050017
2. 河北医科大学附属三院肌电图室,石家庄,050051 |
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| 基金项目： | 河北省科技厅资助项目 |
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| 摘 要： | 目的探讨高糖环境中人肾小球系膜细胞（human mesangial cells,HMC）SREBP-1、FAS表达。方法体外培养HMC细胞,随机分为正常糖组、高糖组,免疫细胞化学、Western Blot和RT-PCR方法检测固醇调节元件结合蛋白1（sterol regulatory element binding protein-1,SREBP-1）和脂肪酸合酶（fatty acid synthase,FAS）表达。采用脂质体转染技术将特异性SREBP-1质粒引入细胞内并进行表达,进一步采用RT-PCR方法检测脂肪酸合酶FAS的表达。结果与正常糖组比较,高糖培养的人肾小球系膜细胞固醇调节元件结合蛋白1前体和成熟体以及FAS mRNA表达均升高,差异有统计学意义。质粒转染后HMC细胞经免疫组化和Western blot检测证实特异性质粒能够在细胞内高表达SREBP-1蛋白,进一步对FAS的检测证实了FAS mRNA表达升高。结论高糖可诱导人肾小球系膜细胞固醇调节元件结合蛋白1和FAS表达增强且SREBP-1和FAS之间存在有直接关系。
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| 关 键 词： | 高糖 人肾小球系膜细胞 固醇调节元件结合蛋白1 脂肪酸合成酶 |
EFFECT OF HIGH GLUCOSE ON THE EXPRESSION OF SREBP-1 AND FAS IN HUMAN MESANGIAL CELLS |
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| Hao Jun,Zhu Lin,Zhao Song,Liu Wei,Yao Hongye,Liu Shuxia,Duan Huijun.EFFECT OF HIGH GLUCOSE ON THE EXPRESSION OF SREBP-1 AND FAS IN HUMAN MESANGIAL CELLS[J].Chinese Journal of Histochemistry and Cytochemistry,2009,18(2):156-160. |
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| Authors: | Hao Jun Zhu Lin Zhao Song Liu Wei Yao Hongye Liu Shuxia Duan Huijun |
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| Institution: | 1Department of Pathology;Hebei Medical University;Hebei 050017;China;2Department of Electromyography of The 3rd Affiliated Hospital of Hebei Medical University;HeBei 050051;China |
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| Abstract: | Objective To investigate the effect of high glucose on SREBP-1 and FAS in human mesangial cells.Methods HMC cells were cultured using the 1640 medium supplemented with 10% FBS that was divided into 4 groups: normal glucose group,high glucose group,pcDNA3.1 plasmid group and pcDNA3.1-SC1 plasmid group.The expression of sterol regulatory element binding protein-1(SREBP-1) was detected with immunocytochemistry and Western Blot.FAS mRNA was detected by RT-PCR.Results Immunocytochemistry showed that high glucose... |
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| Keywords: | High glucose Human mesangail cell （HMC） Sterol regulatory element binding protein-1 （SREBP-1） FAS （fatty acid synthase） |
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