Furrow-specific endocytosis during cytokinesis of zebrafish blastomeres |
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Authors: | Feng Bo Schwarz Heinz Jesuthasan Suresh |
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Institution: | Laboratory of Fish Developmental Genetics, Institute of Molecular Agrobiology, National University of Singapore, 1 Research Link, NUS, 117604, Singapore. |
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Abstract: | Mutations affecting endocytosis, such as those in clathrin and dynamin, unexpectedly cause defects in cytokinesis in a number of organisms. To explore the relationship between endocytosis and cytokinesis, we used the relatively large cells of the transparent zebrafish embryo. Using fluorescent markers for fluid-phase as well as plasma membrane uptake, we demonstrate that cytokinesis involves furrow-specific endocytosis. Clathrin-coated pits are visible near the furrow in ultrathin sections, while immunolabeling demonstrates that clathrin and caveolin are localized to the cleavage furrow. Hence, it is likely that both clathrin- and caveolae-mediated endocytosis occurs at the furrow during cytokinesis. Dynamin II is also localized to the furrow and may mediate furrow-specific endocytosis. Treatment of embryos with chlorpromazine or with methyl-beta-cyclodextrin, both of which inhibit endocytosis, prevents the normal completion of cytokinesis. These data suggest that furrow-specific endocytosis is an integral part of cytokinesis. |
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Keywords: | cytokinesis endocytosis clathrin caveolin Danio rerio |
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