MAPK-dependent expression of p21(WAF) and p27(kip1) in PMA-induced differentiation of HL60 cells |
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Authors: | Das D Pintucci G Stern A |
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Institution: | Department of Pharmacology, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA. |
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Abstract: | Treatment of HL60 cells with phorbol 12-myristate 13-acetate (PMA) results in growth arrest and differentiation towards the macrophage lineage. PMA-induced changes are easily monitored by morphological changes while cells in suspension start adhering onto substrate. PMA induces rapid activation of the extracellular signal-regulated kinases (ERKs). Activation of the ERK pathway is essential to PMA-induced differentiation of HL60 cells. PMA also induces the expression of the cyclin-dependent kinase inhibitors p21(WAF) and p27(kip1), which is modulated by the use of an inhibitor of the ERK cascade. This implies that a link exists between ERK activation and p21(WAF) and p27(kip1) induction in the process of terminal differentiation. |
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