A method for Pmo25-associated kinase assay in fission yeast: the activity is dependent on two gC kinases Nak1 and Sid1 |
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| Authors: | Kume Kazunori Goshima Tetsuya Miyahara Kohji Toda Takashi Hirata Dai |
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| Affiliation: | Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Japan. |
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| Abstract: | In fission yeast, the conserved proteins, MO25/Pmo25, GC kinase/Nak1, Furry/Mor2, NDR kinase/Orb6, and Mob2, constitute the morphogenesis Orb6 network (MOR). Previously we showed that Pmo25 functions as an upstream component of MOR and that it plays a connecting role between the septation initiation network (SIN) and MOR. Here we establish a Pmo25-associated kinase assay and show that the activity is dependent on Nak1/MOR and Sid1/SIN. |
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