Kip3, the yeast kinesin-8, is required for clustering of kinetochores at metaphase |
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Authors: | Megan M Wargacki Jessica C Tay Eric G Muller Charles L Asbury Trisha N Davis |
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Institution: | 1.Department of Biochemistry; University of Washington; Seattle, WA USA;2.Department of Physiology and Biophysics; University of Washington; Seattle, WA USA |
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Abstract: | In Saccharomyces cerevisiae, chromosome congression clusters kinetochores on either side of the spindle equator at metaphase. Many organisms require one or more kinesin-8 molecular motors to achieve chromosome alignment. the yeast kinesin-8, Kip3, has been well studied in vitro but a role in chromosome congression has not been reported. We investigated Kip3''s role in this process using semi-automated, quantitative fluorescence microscopy and time-lapse imaging and found that Kip3 is required for congression. Deletion of KIP3 increases inter-kinetochore distances and increases the variability in the position of sister kinetochores along the spindle axis during metaphase. Kip3 does not regulate spindle length and is not required for kinetochore-microtubule attachment. Instead, Kip3 clusters kinetochores on the metaphase spindle by tightly regulating kinetochore microtubule lengths.Key words: Cin8, cluster, GFP-tubulin, kinesin-5, kinesin-8, kinetochore, Kip3, metaphase, microtubule, mitosis, spindle |
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