Evidence for the binding of the carcinogen 3-methylcholanthrene to both the purine and the pyrimidine bases of hamster fibroblast deoxyribonucleic acid (Short Communication) |
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| Authors: | Peter A. Jones Wieland Gevers Arthur O. Hawtrey |
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| Affiliation: | Unit for Molecular and Cellular Cardiology of the University of Stellenbosch and the Medical Research Council, P.O. Box 63, Tiervlei, South Africa;Department of Biochemistry, University of Rhodesia, Box MP 167, Mount Pleasant, Rhodesia |
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| Abstract: | The binding of [(3)H]3-methylcholanthrene to the DNA of hamster fibroblasts was studied by using chemical methods for DNA degradation. DNA depurinated by mild acid hydrolysis released approximately half of the radioactivity at the same rate as the purine bases, but the resulting apurinic acid still contained radioactive carcinogen. |
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