| 黑木耳Au185菌株一个SCAR标记的建立 |
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| 引用本文： | 马庆芳,张丕奇,戴肖东,韩增华,孔祥辉,刘佳宁,张介驰.黑木耳Au185菌株一个SCAR标记的建立[J].菌物研究,2009,7(2):104-108,115. |
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| 作者姓名： | 马庆芳 张丕奇 戴肖东 韩增华 孔祥辉 刘佳宁 张介驰 |
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| 作者单位： | 黑龙江省科学院微生物研究所,哈尔滨,150010 |
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| 基金项目： | 黑龙江省科学院创新基金项目 |
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| 摘 要： | 在对60个供试黑木耳菌株RAPD指纹图谱进行分析的基础上获得了黑木耳菌株Au185的RAPD特异性标记,将其克隆、测序,应用Primer5.0软件设计相应的特异引物对SC38-1,将RAPD特异性标记转化为黑木耳菌株Au185的稳定方便的SCAR标记,可快速、准确地鉴定出黑木耳菌株Au185。
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| 关 键 词： | 黑木耳Au185菌株 RAPD特异性标记 SCAR标记 |
A SCAR Marker for Auricularia auricula Strain Au185 |
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| MA Qing-fang,ZHANG Pi-qi,DAI Xiao-dong,HAN Zeng-hua,KONG Xiang-hui,LIU Jia-ning,ZHANG Jie-chi.A SCAR Marker for Auricularia auricula Strain Au185[J].Journal of Fungal Research,2009,7(2):104-108,115. |
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| Authors: | MA Qing-fang ZHANG Pi-qi DAI Xiao-dong HAN Zeng-hua KONG Xiang-hui LIU Jia-ning ZHANG Jie-chi |
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| Institution: | ( Iistitute of Microbiology, Heilongfiang Academy of Sicences, Harbin 150010, China ) |
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| Abstract: | The RAPD analysis was conducted on 60 strains of Auricularia auricula. A specific RAPD marker was found in strain Au185 and converted into a stable SCAR （Sequence Characterized Amplified Region） marker by design of specific primers of SC38-1-FP and Sc38-1-RP. This pair of primers can identify Auricularia auricular strain Au185 more accurately and rapidly by using PCR method. |
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| Keywords: | Auricularia auricula Au185 specific RAPD marker SCAR marker |
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