Interleukin-1 alpha and beta induce interleukin-1 beta gene expression in human dermal fibroblasts |
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| Authors: | A Mauviel N Temime D Charron G Loyau J P Pujol |
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| Affiliation: | Laboratoire de Biochimie du Tissu Conjonctif, C.H.U. C?te de Nacre, Caen, France. |
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| Abstract: | The ability of the two forms of interleukin-1, IL-1 alpha and IL-1 beta, to induce IL-1 beta gene expression in human skin fibroblasts was studied in vitro, using Northern blot hybridization. Both recombinant IL-1 alpha and IL-1 beta caused a dramatic increase in IL-1 beta mRNA levels, IL-1 alpha being more efficient than IL-1 beta. Blockage of the prostaglandin synthesis by indomethacin reduced the basal level of IL-1 beta mRNA in control cultures and decreased also the stimulatory effect exerted by both IL-1s on IL-1 beta gene expression. These data suggest that IL-1 and prostaglandin (mainly PGE2) may act synergistically to stimulate IL-1 gene expression in dermal fibroblasts, contributing as a local amplifier system to the alterations of connective tissue in inflammatory processes. |
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