Chromium reduction in Pseudomonas putida |
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Authors: | Y Ishibashi C Cervantes S Silver |
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Institution: | Department of Microbiology and Immunology, University of Illinois, College of Medicine, Chicago 60680. |
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Abstract: | Reduction of hexavalent chromium (chromate) to less-toxic trivalent chromium was studied by using cell suspensions and cell-free supernatant fluids from Pseudomonas putida PRS2000. Chromate reductase activity was associated with soluble protein and not with the membrane fraction. The crude enzyme activity was heat labile and showed a Km of 40 microM CrO4(2-). Neither sulfate nor nitrate affected chromate reduction either in vitro or with intact cells. |
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