采用质粒DNA研究人结缔组织生长因子（hCTGF）对骨损伤修复作用的研究

摘要：目的 研究人结缔组织生长因子（hCTGF）对细胞的增殖作用及其骨损伤促愈合的生物学作用。方法 利用真核基因导入系统，转染具有分化潜能的成纤维细胞，并导入骨折实验动物模型，利用分子生物学和临床骨科技术检测分析，获得数据。结果 明确了hCTGF对细胞的增殖作用，表明了hCTGF以基因治疗为手段具有修复骨损伤的生物学活性，为该因子在临床上治疗骨损伤提供新的活性因子和新的治疗方法。

Study of hCTGF Repair on Bone Injury

Object: To study the proliferation of hCTGF on cells and its biological function on bone injury healing.Methods: The fibroblast with potential differentiation was transfected by eukaryotic gene delivery system and then transferred into the experimental animal model with bone fracture.The data were collected by molecular biological and clinical orthopedic technique detection analysis.Results: The results demonstrated an obvious proliferation of hCTGF on cells,suggesting that hCTGF have the biological activity of repairing bone injury via gene therapy.The results provide a new activity factor and treatment approach for bone injury in clinics.