| 双酚A对中国林蛙生精细胞凋亡和Bax、Bcl-2表达的影响 |
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| 引用本文： | 林胜男,张育辉,李国庆,王宏元. 双酚A对中国林蛙生精细胞凋亡和Bax、Bcl-2表达的影响[J]. 动物学报, 2008, 54(5) |
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| 作者姓名： | 林胜男 张育辉 李国庆 王宏元 |
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| 作者单位： | 陕西师范大学生命科学学院,西安,710062 |
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| 基金项目： | 国家自然科学基金资助项目(No.30770243)~~ |
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| 摘 要： | 为探讨双酚A(BPA)对两栖动物生精细胞凋亡及相关蛋白Bax和Bcl-2表达的影响.将雄性中国林蛙(Rana chensinensis)分别暴露于10-7、10-6、10-5 mol/L BPA水体中持续3 d、5 d、7 d,取其精巢组织.用原位末端转移酶法(TUNEL)和甲基绿-派诺宁法(Methyl Green-Pyronine)检测生精细胞凋亡,用免疫组织化学方法检测生精细胞的Bax和Bcl-2表达.结果显示,各BPA处理组中国林蛙生精细胞凋亡指数(Apoptotic index,AI)均显著高于对照组,10-6 mol/L与10-7 mol/L BPA处理组生精细胞的AI差异不显著,10-5 mol/L BPA处理组生精细胞的AI与前两组相比显著增高;在同一BPA浓度处理组,生精细胞AI随处理时间的延长而增高.与对照组相比,各BPA处理组Bax表达上调,Bcl-2表达下调,差异均显著;生精细胞AI与Bax/Bcl-2表达呈正相关.这些结果提示,BPA可导致中国林蛙的生精细胞凋亡,而生精细胞凋亡的发生与Bax/Bcl-2表达比值的变化密切相关.
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| 关 键 词： | 中国林蛙 双酚A 生精细胞 细胞凋亡 |
Effects of Bisphenol A on apoptosis of spermatogenic cells and expression of Bax and Bcl-2 proteins in the Siberian frog Rana chensinensis |
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| LIN Sheng-Nan,ZHANG Yu-Hui,LI Guo-Qing,WANG Hong-Yuan. Effects of Bisphenol A on apoptosis of spermatogenic cells and expression of Bax and Bcl-2 proteins in the Siberian frog Rana chensinensis[J]. Acta Zoologica Sinica, 2008, 54(5) |
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| Authors: | LIN Sheng-Nan ZHANG Yu-Hui LI Guo-Qing WANG Hong-Yuan |
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| Affiliation: | LIN Sheng-Nan,ZHANG Yu-Hui,LI Guo-Qing,WANG Hong-YuanCollege of Life Science,Shaanxi Normal University,Xi'an 710062,China |
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| Abstract: | In order to investigate the effects of bisphenol A(BPA)on apoptosis and the expression levels of Bax and Bcl-2 protein in amphibian spermatogenic cells,we treated Rana chensinensis with concentrations of 10-7,10-6,10-5mol/L BPA respectively.Testes were removed after R.chensinensis had been treated for 3,5 or 7 days.Apoptotic cells were examined by TUNEL and Methyl Green-Pyronine.The expression levels of Bax and Bcl-2 protein in spermatogenic cells were detected by immunohistochemical staining.The results sh... |
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| Keywords: | Bcl-2 Bax |
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