应用ESR检测探讨急性热暴露大鼠肝脏氧化还原状态的动态变化

目的:通过电子顺磁自旋共振技术(ESR)动态观察大鼠在过热条件下肝脏的氧化还原状态.方法:将52只雄性Wistar大鼠随机分成4组:①加温组:麻醉后进行整体加温到直肠温达(43.0±0.5)℃,持续15 min;②对照组:只进行麻醉处理;③,MPG预处理组:用抗氧化剂MPG预处理后,再进行与上述①同样条件的加温处理;④非MPG预处理组:在③中用生理盐水代替MPG.经过以上处理后在不同时间点取肝脏制备组织匀浆,测定ESR波谱.结果:与对照组比较,加温组热暴露处理后记录的ESR波谱振幅-时间直线斜率增大,2 h达最大值.以后逐渐恢复,24 h接近对照组水平.经抗氧化剂预处理上述反应减弱.结论:过热能诱导肝脏产生活性氧,增强其氧化还原反应.

Redox dynamics in hyperthermia in rat livers by ESR spectroscopy

Aim:The redox dynamics of the rat liver in the whole body hyperthermia was measured by the decay rate of the ESR spectra used carbamoyl PROXYL as a spin probe.Methods:Fifty two Wistar male rats were randomly assigned to four groups and each group was subjected to only one of the following treatments:heat stress(HS group),anesthesia without hyperthermia(control group),pretreatment with MPG(MPG HS group) and pretreatment with saline(No MPG group).The solution of carbamoyl PROXYL was added to rat liver homogenate from 0,1,2,6 and 24 h after heating at concentration of 0.1 mmol/L.sequentially the ESR spectra of the samples were immediately recorded by X band ESR spectrometer.Results:Compared with the control group,the signal decay rate was increased gradually after heat treatment,and attained the maximum at 2 h,and then returned to the normal level at 24 h.This signal intensity effect was reduced by MPG pretreatment.Conclusion:Generation of reactive oxygen species in liver may be induced by heat stress.