块菌(松露)宿主可培养内生菌群落结构与多样性研究

探究四川凉山彝族自治州块菌主产区华山松内生菌群的结构及多样性。在会东县选取4个点(新田乡、新云乡、淌塘镇、雪山乡)的块菌宿主华山松的根、茎、叶为实验材料,通过不同培养基分离样品根、茎、叶的内生细菌、真菌、放线菌,DNA分子鉴定分离菌株的种属,最终分离得到细菌46株,其中欧文氏菌属(Erwinia)5株,沙门氏菌属(Salmonell)1株,杆菌属(Bacillus)22株,葡萄球菌属(Staphylococcus)2株,假单胞菌属(Pseudomonas)2株,类芽胞杆菌属(Paenibacillus)2株,布丘氏菌属(Buttiauxella)2株,肠杆菌属(Enterobacte)3株,爱文氏菌属(Ewingella)1株,泛菌属(Rahnella)1株,拉恩氏菌属(Rahnella Izard)2株,其他属3株;真菌19株,均为子囊菌门(Ascomycota),其中青霉属(Penicillium)4株,疱霉属(Phoma)1株,子囊菌门未知菌1株,篮状菌属(Cladosporium)1株,分枝孢子菌属(Sydowia)1株,曲霉属(Aspergillus)1株,其他属10株;放线菌33株,为链霉菌属(Streptomyces)22株,短小杆菌属(Curtobacterium)2株,短杆菌属(Brevibacterium)1株,其他属8株。研究结果表明,来自不同地点、不同植株部位、不同培养基分离得到的块菌宿主华山松内生菌有差异,证实微生物在土壤、块菌、宿主植物之间有着复杂的相互作用,为块菌的人工栽培提供参考。

Structure and Diversity of Cultivable Endophyte from Hosts of Truffle (Pinus armandii)

In order to explore the structure and diversity of endophytic community in the main truffle producing areas of Liangshan Autonomous Prefecture of the Yi Nationalities, Sichuan Province. Roots, stems, and leaves of Pinus armandii as experimental materials from four point (Xintian, Xinyun, Tangtang, and Xueshan Townships) in Huidong County to insolate their endophytic bacteria, fungi, and actinomycetes with different media, and identify their genus belonging species by DNA sequencing. The results showed that a total of 46 strains of bacteria were isolated, including 5 Erwinia strains, 1 Salmonell strain, 22 Bacillus strains, 2 Staphylococcus strains, 2 Pseudomonas strains, 2 Paenibacillus strains, 2 Buttiauxella strains, 3 Enterobacte strains, 1 Ewingella strain, 1 Rahnella strain, 2 RahnellaIzard strains, and 3 strains of other genera. Nineteen strains of fungi belonged to Ascomycota, including 4 strains of Penicillium, 1 Phoma strain, 1 Cladosporium strain, 1 Sydowia strain, 1 Aspergillus strain, unknown 1 strain of bacteria affiliated with Ascomycetes, and 10 strains of other genera. In addition, thirty three strains were affiliated with actinomycetes. According to analyses, twenty two strains were Streptomyces, two were Curtobacterium, one was Brevibacterium and 8 strains belonged to other genera. The result indicated that the Endophyte from Pinus armandii of truffle have differences in different sites, parts of plants and isolated from different media. It was proved that microorganisms have complex interaction among soil, truffles and host plants, This research laid a certain foundation for artificial cultivation of truffles.