应用ELISA和ELFA定量检测牛奶中葡萄球菌肠毒素A方法的建立

探讨了Tecra SEs SET(ELISA法)和Vidas SET2(ELFA法)两种葡萄球菌肠毒素定性检测试剂盒用于定量检测牛奶中葡萄球菌肠毒素A(SEA)的可行性。根据GB/T27404-2008《实验室质量控制规范食品理化检测》的要求,对两种方法应用于定量检测时的检出限、校正曲线范围、相关系数和加标回收率指标进行了分析比较。实验结果显示,Tecra SEs SET对牛奶中SEA的检出限为0.79 ng/mL,校正曲线范围为0.79~10 ng/mL,相关系数r=0.997,SEA加标浓度为0.80、2.5和10 ng/mL时的回收率分别为110%、81%和100%。Vidas SET2的检出限为0.09 ng/mL,校正曲线范围为0.09~1.0 ng/mL,相关系数r=0.998,SEA加标浓度为0.1、0.25和1.0 ng/mL时的回收率分别为90%、95%和104%。上述结果结合对阳性样品的检测表明:这两种定性检测试剂盒能满足牛奶中SEA定量检测的要求。

Quantitative Detection of the Staphylococcal Enterotoxin A in Milk Using ELISA and ELFA Kit

The aim of this study was to evaluate the feasibility for the quantitative detection of the staphylococcal enterotoxin A(SEA) concentration in milk with two qualitative detection kits: Tecra SEs SET (ELISA method) and Vidas SET2 (ELFA method). The detection limit, range of calibration curve, correlation coefficient and spike recovery of both kits were compared and analyzed according to the requirements in GB/T27404 2008 "Criterion on quality control of laboratories Chemical testing of food". The experiment results showed that the detection limit of the Tecra Ses SET for the detection of SEA in milk was 0.79 ng/mL with valid range of calibration curve ranged from 0.79-10 ng/mL. The correlation coefficient was 0.997. The spike recovery of Tecra Ses SET at the SEA concentration of 0.80, 2.5 and 10 ng/mL were 110%, 81%, and 100% respectively. The detection limit of Vidas SET2 was 0.09 ng/mL with valid range of calibration curve ranged from 0.09-1.0 ng/mL. When correlation coefficient was 0.998 the spike recoveries of Vidas SET2 at SEA concentration were 0.1, 0.25, and 1.0 ng/mL were 90%, 95%, and 104% respectively. The above results combined with the detection of positive samples indicated that these two qualitative detection kits meet the requirement for quantitative detection of SEA in milk.