| 运用萎锈灵抗性基因构建香菇聚乙二醇介导的遗传转化方法 |
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| 引用本文： | 鲍大鹏,杨瑞恒,王莹,茅文俊,汪滢,李燕.运用萎锈灵抗性基因构建香菇聚乙二醇介导的遗传转化方法[J].微生物学通报,2018,45(8):1824-1828. |
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| 作者姓名： | 鲍大鹏 杨瑞恒 王莹 茅文俊 汪滢 李燕 |
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| 作者单位： | 国家食用菌工程技术研究中心农业部南方食用菌资源利用专业重点实验室上海市农业科学院食用菌研究所 |
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| 基金项目： | 沪农科攻字(2015)第6-2-1号(G2015060201) |
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| 摘 要： | 【背景】萎锈灵抗性基因作为筛选标记在植物和真菌中得到广泛应用。【目的】构建可以使用萎锈灵作为筛选标记的香菇遗传转化技术。【方法】利用溶壁酶消化培养4 d的香菇菌株411-4的菌丝体获得原生质体,加入适量pL-cbx质粒和聚乙二醇溶液,混合物涂布于再生筛选培养基上,培养后挑选菌落进行验证试验。【结果】在原生质体数目108个和添加4μg质粒DNA的情况下,得到了40个抗性转化子。利用PCR实验和转代实验对转化子进行验证,结果显示38个转化子的抗性可稳定遗传,表明萎锈灵抗性基因整合进入了供试菌株的基因组中。【结论】利用香菇411-4菌株建立了一套运用萎锈灵抗性基因作为分子标记的遗传转化技术体系。
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| 关 键 词： | 香菇，聚乙二醇介导的原生质体转化，萎锈灵 |
PEG-mediated genetic transformation of Lentinula edodes by using carboxin resistant gene as selective marker |
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| BAO Da-Peng,YANG Rui-Heng,WANG Ying,MAO Wen-Jun,WANG Ying and LI Yan.PEG-mediated genetic transformation of Lentinula edodes by using carboxin resistant gene as selective marker[J].Microbiology,2018,45(8):1824-1828. |
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| Authors: | BAO Da-Peng YANG Rui-Heng WANG Ying MAO Wen-Jun WANG Ying and LI Yan |
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| Institution: | National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China,National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China,National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China,National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China,National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China and National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China |
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| Abstract: | |
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| Keywords: | Lentinula edodes PEG mediated protoplast transformation Carboxin |
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